Bio-Technology – Important Question Bank for JKBOSE (Summer Zone & Winter Zone) HSC Examination 2016

Long Question 5 Mark:

1.Write short notes on the following.

• Secondary structure of protein.
• Tertiary structure of protein.
• Recombinant DNA molecule.
• Restriction enzymes
• Human genome project, its significance.
• Bioinformatics and applications.

2. Define protein engineering? Write down steps involved in protein Engineering?

3. What is DNA library? Write down methods involved in it.

4. What is Southern blotting technique? Write down the various steps involved in it?

5. What do mean by PCR? Describe in detail?

6. Define and discuss structural genome and functional genome?

7. Define recombinant DNA technology? Write down steps involved in it.

8. Define the following

• Cloning vector.
• Expression vector.
• Binary vector.

Short Questions 3 Mark:

9. What are conjugated proteins? Give two examples.

10. What are plasmids? Write its importance.

11. Define proteins. Give examples of secondary protein.

12. What are hydrophobic bonds, where they are found?

13. Write down the application of PCR.

14. Define Genome Library.

15. What are DNA PROBES?

Very short Question 2 Mark:

16. Name various types of interaction (Non-covalent bonds) found in proteins.

17. Name four protein based products.

18. What is transformation?

19. Where was NCB published?

20. How gene bank was build.

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